PUMA (I17) Antibody-Delta Biolabs

Product Info
Background	The Bcl-2 family proteins can function to either suppress or promote cell death and are characterized by the presence of up to four conserved amino acid motifs, termed Bcl-2 homology (BH) domains (1). The BH3 function has been further revealed by an emerging group of BH3-only proteins, that includes Bik, Bad, Bid, Bim, Noxa, and the newest member PUMA or bbc3 (1&2). The PUMA gene is a target of p53 transcriptional activation and encodes two BH3 domain-containing proteins (PUMA-alpha and PUMA-beta) (3&4). The activities of PUMA-alpha and PUMA-beta are similar; they bind to Bcl-2, localize to the mitochondria to induce cytochrome c release, and activate extremely rapid apoptosis (3&4).
Origin	PUMA (I17) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to an internal domain of human PUMA.
*Product Citations*	1.) Goldberg Z, Levav Y, Krichevsky S, Fibach E, Haupt Y. 2004. Treatment of chronic myeloid leukemia cells with imatinib (STI571) impairs p53 accumulation in response to DNA damage. Cell Cycle. Sep;3(9):1188-95.
Product Details	Each vial contains 200 �g/ml of affinity purified rabbit IgG PUMA (I17) DB043, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Competition Studies	A blocking peptide is also available, DB043P, for use in competition studies. Each vial contains 100 mg of peptide in 0.5 ml PBS with 0.1% sodium azide and 100 mg BSA.
Form	200 �g/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Immunogen	Synthetic peptide mapping to an internal domain of human PUMA.
Specificity	PUMA (I17) DB043 reacts with PUMA of mouse, rat, and human origin
Use	Western blotting. Western blotting starting dilution 1:200.
Storage	Store this product at 4� C, do not freeze. The product is stable for one year from the date of shipment.
References	1. Han J., Flemington C., Houghton A.B., Gu Z., Zambetti G.P., Lutz R.J., Zhu L., Chittenden T. 2001. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. PNAS 98(20):11318-11323. 2. Schuler M., Green D.R. 2001. Mechanisms of p53-dependent apoptosis. Biochem Soc Trans 29(Pt6):684-688. 3. Nakano K., Vousden K.H. 2001. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell 7(3):683-694. 4. Yu J., Zhang L., Hwang P.M., Kinzler K.W., Vogelstein B. 2001. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell 7(3):673-682.