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VEGF (VG-1) Antibody DB096 0.100 mg/ml $275.00

Datasheet 

For technical service please call (800) 595 1994
Product Info
Background The vascular endothelial growth factor (VEGF) family currently includes VEGF (VEGF-A), VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF (1). VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis (2). Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process (3&4). To date 5 different isoforms of VEGF have been described, VEGF 121, VEGF 145, VEGF 165, VEGF 165b, and VEGF 189(4&5). These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells (5). 
Origin VEGF (VG-1) is provided as a mouse monoclonal IgG1 antibody derived from BALB/C mice immunized with recombinant VEGF. 
Product Details Each vial contains 100 g/ml of mouse monoclonal IgG1 VEGF DB096 (VG-1), in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Form 100 g/ml mouse monoclonal IgG1 in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Immunogen Recombinant VEGF 
Specificity VEGF DB096 (VG-1) detects the 121, 165, and 189 VEGF isoforms of Human origin 
Use Western blotting, ELISA, immunohistochemistry (including paraffin-embedded sections). Western blotting starting dilution 1:200, immunohistochemistry starting dilution 1:50. 
Storage Store this product at 4 C, do not freeze. The product is stable for one year from the date of shipment. 
References 1. Matsumoto T, Claesson-Welsh L. 2001. VEGF receptor signal transduction. Sci STKE 112:RE21.
2. Shibuya M. 2001. Structure and function of VEGF/VEGF-receptor system involved in angiogenesis. Cell Struct Funct 26(1):25-35.
3. Hasan J, Jasyson GC. 2001. VEGF antagonists. Expert Opin Biol Ther 1(4):703-18.
4. Bates DO, Cui TG, Doughty JM, Winkler M, Sugiono M, Shields JD, Peat D, Gillatt D, Harper SJ. 2002. VEGF165b, an inhibitory splice variant of vascular endothelial growth factor, is down-regulated in renal cell carcinoma. Cancer Res 62(14):4123-31.
5. Neufel G, Cohen T, Gengrinovitch S, Poltorak Z. 1999. Vascular endothelial growth factor (VEGF) and its receptors. FASEB 13(1):9-22.
 

For Technical service please call +1-800-595-1994
Delta Biolabs+1-408-846-6650 • fax:+1-408-846-6645 • Copyright © 2001.Allrights reserved.
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