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Product Catalog# Quantity Unit Price Buy
beta Gal (FL2) Antibody DB087 0.200 mg/ml $210.00

Datasheet 
Datasheet 

For technical service please call (800) 595 1994
Product Info
Background The Escherichia coli lacZ gene encodes the enzyme beta-galactosidase. Beta-galactosidase is a large 464 kDa tetramer when assayed by western blot under nonreducing conditions and 116 kDa monomer under reducing conditions. The beta-galactosidase activity allows for the quick determination of cells expressing the lacZ gene. The lacZ gene is used as a reporter construct for determination of transfection efficiency as well as histochemical localization following transfection of eukaryotic cells. The lacZ gene product beta-galactosidase catalyzes the hydrolysis of the substrate 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) to produce a blue color that is easily visualized with a microscope. 
Origin Rabbits were immunized with purified beta galactosidase from Escherichia coli. Antibodies were affinity purified using purified beta-galactosidase immobilized on a solid phase. 
Product Details Each vial contains 200 µg/ml of affinity purified rabbit IgG, beta Gal DB087 (FL2), in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Form 200 µg/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Immunogen purified beta galactosidase from Escherichia coli 
Use Beta-Galactosidase (FL2) DB087 is recommended for use by western blotting and immunohistochemistry. The suggested western blotting starting dilution is at 1:400. 
Specificity Will detect beta galactosidase fusion proteins 
Storage Store this product at 4º C, do not freeze. The product is stable for one year from the date of shipment. 

For Technical service please call +1-800-595-1994
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