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caspase-9 p10 (I18) Antibody DB081 0.200 mg/ml $200.00


For technical service please call (800) 595 1994
Product Info
Background The activation of the initiator caspase, caspase-9, is regulated by the apoptosome, which is composed of cytochrome c bound to Apaf-1 and dATP (1&2). The apoptosome responds to a diverse array of apoptotic stimuli that initiate the caspase cascade that ultimately results in cell death. The cascade of events starts with the proteolytic cleavage of caspase-9, that then cleaves downstream caspases, caspase-3, -6, and ?7 (1). The apoptotic initiating activity of caspase-9 can be regulated by XIAP, X-linked inhibitor-of-apoptosis protein; through the binding of caspase-9 at it amino terminus while it is bound to the apoptosome (3 & 4). This inhibition of caspase-9 activity has been shown to be relieved by Smac/DIABLO through its interaction with XIAP (3). 
Origin caspase-9 p10 (I18) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to an internal domain of human caspase-9 p10 subunit. 
Product Details Each vial contains 200 g/ml of affinity purified rabbit IgG, caspase-9 p10 (I18) DB081, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Competition Studies A blocking peptide is also available, DB081P, for use in competition studies. Each vial contains 0.100 mg of peptide in 0.5 ml PBS with 0.1% sodium azide and 100 mg BSA. 
Form 200 g/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Immunogen Synthetic peptide mapping to an internal domain within the p10 subunit of human caspase-9 
Specificity Mouse, rat, and human caspase-9 (p10) and caspase-9 precursor 
Use Mouse, rat, and human caspase-9 p10 and caspase-9 precursor 
Storage Store this product at 4 C, do not freeze. The product is stable for one year from the date of shipment. 
References 1. Kuida K. 2000. Caspase-9. Int J Biochem Cell Biol. 32(2):121-124.
2. Potokar M, Milisav I, Kreft M, Stenovec M, Zorec R. 2003. Apoptosis triggered redistribution of caspase-9 from cytoplasm to mitochondria. FEBS Lett. 544(1-3):153-159.
3. Srinivasula SM, Hegde R, Saleh A, Datta P, Shiozaki E, Chai J, Lee RA, Robbins PD, Fernandes-Alnemri T, Shi Y, Alnemri ES. 2001. A conserved XIAP-interaction motif in caspase-9 and Smac/DIABLO regulates caspase activity and apoptosis. Nature 410(6824):112-116.
4. Zou H, Yang R, Hao J, Wang J, Sun C, Fesik SW, Wu JC, Tomaselli KJ, Armstrong RC. 2003. Regulation of the Apaf-1/caspase-9 apoptosome by caspase-3 and XIAP. J Biol Chem. 278(10):8091-8098.

For Technical service please call +1-800-595-1994
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Protein Kinases| Secondary Antibodies| Signaling Intermediates| Transcription Regulation