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cyclin B1 (C20) Antibody DB013 0.200 mg/ml $190.00


For technical service please call (800) 595 1994
Product Info
Background During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by activating Cdks (1,2). Cyclin A regulates at least two cdks, Cdc2 p34 and Cdk2, and is believed to be necessary for progression through S phase and is active during the G2-M transition (1&3,4). Cyclin B1 also associates with Cdc2 p34 and this complex is requisite for the G2-M progression. The Cyclin B1/Cdc2 p34 complex is known as MPF, M-phase kinase, maturation-promoting factor, or M-phase specific histone kinase, is widely accepted as the trigger of mitosis in all organisms (5-9). 
Origin Cyclin B1 (C20) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the carboxy terminus of human cyclin B1. 
Product Details Each vial contains 200 g/ml of affinity purified rabbit IgG, cyclin B1 (C20) DB013, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Competition Studies A blocking peptide is also available, DB013P, for use in competition studies. Each vial contains 0.100 mg of peptide in 0.5 ml PBS with 0.1% sodium azide and 100 mg BSA. 
Form 200 g/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Immunogen Synthetic peptide mapping to the carboxy terminus of human cyclin B1. 
Western blot analysis of cyclin B1 p58 expression in HeLa whole cell lysates. 
Use Cyclin B1 (C20) DB013 reacts with cyclin B1 of mouse, rat, and human origin by western blotting and immunohistochemistry. Western blotting starting dilution: 1:200. 
Storage Store this product at 4 C, do not freeze. The product is stable for one year from the date of shipment. 
References 1. Bandara LR, Adamczewski JP, Zamanian M, Poon RY, Hunt T, Thangue NB. 1992. Cyclin A recruits p33cdk2 to the cellular transcription factor DRTF1. J Cell Sci Suppl 16: 77-85.
2. Desdouets C, Sobczak-Thepot J, Murphy M, Brechot C. 1995. Cyclin A: Function and expression during cell proliferation. Prog Cell Cycle Res 1:115-123.
3. Brechot C. 1993. Oncogenic activation of cyclin A. Curr Opin Genet Dev 3(1): 11-18.
4. Goldstone S, Pavey S, Forrest A, Sinnamon J, Gabrielli B. 2001. Cdc25-dependent activation of cyclin A/cdk2 is blocked in G2 phase arrested cells independently of ATM/ATR. Oncogene 20(8): 921-932.
5. Liu D, Liao C, Wolgemuth DJ. 2000. A role for cyclin A1 in the activation of MPF and G2-M transition during meiosis of male germ cells in mice. Dev Biol 224(2): 338-400.
6. Hoffman I, Clarke PR, Marcotte MJ, Karsenti E, Draetta G. 1993. Phosphorylation and activation of human cdc25-C by cdc2-cyclin B and its involvement in the self-amplification of MPF at mitosis. EMBO J 12(1): 53-63.
7. Kishimoto T, Okumura E. 1997. In vivo regulation of the entry into M-phase: initial activation and nuclear translocation of cyclin B/Cdc2. Prog Cell Cycle Res 3:241-249.
8. Baratte B, Meijer L, Galaktionov K, Beach D. 1992. Screening for antimitotic compounds using the cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2/cyclin Bcdc13 protein kinase. Anticancer Res 12(3): 873-880.
9. Thron CD. 1994. Theoretical dynamics of the cyclin B-MPF system: a possible role for p13suc1. Biosystems 32(2):97-109.

For Technical service please call +1-800-595-1994
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