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Cdc2 p34 (17) Antibody DB103 0.100 mg/ml $275.00


For technical service please call (800) 595 1994
Product Info
Background Cyclin dependent kinases are key regulators of the progression of the cell cycle. Early in the cell cycle Cdk2, Cdk4, Cdk6 and their associated cyclins regulate the G1 to S phase transition (1, 2). Cdk2 plays a key role in the G1/S and S/G2 transitions through its associations with cyclin D1, cyclin D2, cyclin D3, cyclin E and cyclin A. Cdk4 also forms complexes with the D type cyclins, and is thought to regulate cell growth through the G1 phase of the cell cycle (3-6). The late stages of the cell cycle are regulated by another cyclin dependent kinase, Cdc2 p34. This kinase exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34-cyclin B complex that is required for the G2 to M phase transition (7,8). 
Origin Cdc2 p34 (17) is provided as mouse monoclonal IgG2a corresponding to amino acids 225-230, mapping within a central region of Cdc2 p34 of human origin. 
Product Details Each vial contains 100 g/ml of mouse monoclonal IgG2a Cdc2 p34 (17) DB103, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Form 100 g/ml mouse monoclonal IgG2a in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Immunogen Recognizes an epitope corresponding to amino acids 225-230, mapping within a central region of Cdc2 p34 of human origin. 
Specificity Cdc2 p34 (17) DB103 reacts with Cdc2 p34 of murine, human, and Xenopus origin 
Use Western blotting, immunoprecipitation (as active kinase) and immunohistochemistry (including paraffin-embedded sections). 
Storage Store this product at 4 C, do not freeze. The product is stable for one year from the date of shipment. 
References 1. Ekholm SV, Reed SI. 2000. Regulation of G(1) cyclin-dependent kinases in the mammalian cell cycle. Curr Opin Cell Biology 12(6): 676-684
2. Morisaki H, Ando A, Nagata Y, Pereira-Smith O, Smith JR, Ikeda K, Nakanishi M. 1999. Complex mechanisms underlying impaired activation of Cdk4 and Cdk2 in replicative senescence: roles of p16, p21, and cyclin D1. Exp Cell Research 253(2): 503-510.
3. Tong W, Pollard JW. 1999. Progesterone inhibits estrogen-induced cyclin D1 and cdk4 nuclear translocation, cyclin E- and cyclin A-cdk2 kinase activation, and cell proliferation in uterine epithelial cells in mice. Mol Cell Biology 19(3): 2251-64.
4. Sweeney KJ, Sarcevic B, Sutherland RL, Musgrove EA. 1997. Cyclin D2 activates Cdk2 in preference to Cdk4 in human breast eptithelial cells. Oncogene 14(11): 1329-1340.
5. Chu CY, Lim RW. 2000. Involvement of p27(kip1) and cyclin D3 in the regulation of cdk2 activity during skeletal muscle differentiation. Biochimica Biophysica Acta 1497(2): 175-185.
6. Kishimoto T, Okumura E. In vivo regulation of the entry into M-phase: initial activation and nuclear translocation of cyclin B/Cdc2. 1997. Prog Cell Cycle Res 3:241-249.
7. Morla AO, Draetta G, Beach D, Wang JY. 1989. Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis. Cell 58(1): 193-203.
8. O?Connor PM, Ferris DK, Pagano M, Draetta G, Pines J, Hunter T, Longo DL, Kohn KW. 1993. G2 delay induced by nitrogen mustard in human cells affects cyclin A/cdk2 and cyclin B1/cdc2-kinase complexes differently. J Biological Chemistry 268(11): 8298-8308.

For Technical service please call +1-800-595-1994
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