AP-2alphabeta (A6/2/2) Antibody-Delta Biolabs

Product Info
Background	The AP-2 transcription factor exists in three isoforms, alpha, beta, and gamma that have proven to be essential for gene expression associated with ectodermal development (1,2). The AP-2 isoforms have also been shown to compose the OB2-1 transcription factor (3,4). OB2-1 has been shown to upregulate c-erbB-2 (Neu), which is overexpressed in 25-30% of breast cancers through increased transcription and amplification of the gene (3). All three AP-2 isoforms are capable of binding to the c-erbB-2 promoter as homo or heterodimers (3). One study has found that AP-2alpha and AP-2gamma are expressed at elevated levels in the majority of c-erbB-2 overexpressing mammary tumor lines they examined (3).
Origin	AP-2alphabeta (A6/2/2) is provided as a mouse monoclonal IgG1 derived from a mouse immunized with a carboxy terminal peptide from AP-2?.
Product Details	Each vial contains 100 �g/ml of mouse monoclonal IgG1 AP-2alphabeta (A6/2/2) DB108, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Form	100 �g/ml of mouse monoclonal IgG1 in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Immunogen	A carboxy terminal peptide from AP-2alpha.
Specificity	AP-2�� (A6/2/2) DB108 reacts with AP-2alpha and AP-2beta of human origin
Use	Western blotting, ELISA, immunoprecipitation and Immunohistochemistry (including paraffin-embedded tissue sections) and FACS. Western blotting starting dilution: 1:200.
Storage	Store this product at 4º C, do not freeze. The product is stable for one year from the date of shipment.
References	1. Oyama N, Takahashi H, Tojo M, Iwatsuki K, Iizuka H, Nakamura K, Homma Y, Kaneko F. 2002. Different properties of three isoforms (alpha, beta, and gamma) of transcription factor AP-2 in the expression of human keratinocytes. Arch Dermatol Res. 294(6):273-280. 2. Hilger-Eversheim K, Moser M, Schorle H, Buettner R. 2000. Regulatory roles of AP-2 transcription factors in vertebrate development, apoptosis and cell cycle control. Gene 260(1-2):1-12. 3. Bosher JM, Totty NF, Hsuan JJ, Williams T, Hurst HC. 1996. A family of AP-2 proteins regulates c-erbB-2 expression in mammary carcinoma. Oncogene 13(8): 1701-1707. 4. Bosher JM, Williams T, Hurst HC. 1995. The developmentally regulated transcription factor AP-2 is involved in c-erbB-2 overexpression in human mammary carcinoma. Proc Natl Acad Sci USA 92(3): 744-747.