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Product Info
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Background |
Expression vectors incorporating the sequence encoding the glutathion-S-transferase (GST) protein are very useful constructs for the production and purification of recombinant proteins. The protein of interest is cloned into these expression vectors producing a GST-fusion protein. The GST-tag allows fusion proteins to be efficiently purified from bacterial, yeast, mammalian or insect cell lysates utilizing an affinity matrix containing glutathione. Elution of the purified protein is accomplished under mild, nondenaturing conditions. The GST fusion adds a 26 kDa tag to a recombinant protein, which can be removed when an endopeptidase cleavage site sequence is incorporated between the tag and the protein.
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Origin |
Rabbits were immunized with highly purified glutathione-S-transferase (GST) from Schistosoma japonicum. Antibodies were affinity purified using GST immobilized on a solid support.
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Product Details |
Each vial contains 200 µg/ml of affinity purified rabbit IgG, GST DB086 (FL1), in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
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Form |
200 µg/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
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Immunogen |
highly purified glutathione-S-transferase (GST) from Schistosoma japonicum.
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Specificity |
GST (FL1) DB086 is specific for glutathione-S-transferase (GST) Schistosoma japonicum and GST fusion proteins.
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Use |
GST DB086 (FL1) is recommended for use in Western blotting and immunopreciptiation analysis. At a 1:1,000 dilution the GST DB086 (FL1) works as an effective probe for Western Blotting.
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Storage |
Store this product at 4º C, do not freeze. The product is stable for one year from the date of shipment.
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