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Product Info
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Background |
One of the more established epitope tags is the HA tag, which is a nine amino acid peptide sequence (YPYDVPDYA) present in the human influenza virus hemagglutinin protein. The HA tag has been successfully used in a variety of applications, including Western blotting, immunoprecipitation, and immunflourescence studies.
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*Product Citations* |
1.) Geng X, Tang RH, Law SK, Tan SM. Integrin CD11a cytoplasmic tail interacts with the CD45 membrane-proximal protein tyrosine phosphatase domain 1. Immunology. 2005 Jul;115(3):347-57.
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Origin |
HA (I9) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to
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Product Details |
Each vial contains 200 µg/ml of affinity purified rabbit IgG, HA (I9) DB062, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
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Competition Studies |
A blocking peptide is also available, DB062P, for use in competition studies. Each vial contains 0.100 mg of peptide in 0.5 ml PBS with 0.1% sodium azide and 100 mg BSA.
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Form |
200 µg/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
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Immunogen |
Synthetic peptide mapping to an internal region of the human influenza HA protein, YPYDVPDYA.
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Specificity |
HA (I9) DB062 detects proteins with the HA tag.
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Use |
HA (I9) is recommended for use by western blotting and immunoprecipitation. The suggested western blotting starting dilution is at 1:200.
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Storage |
Store this product at 4º C, do not freeze. The product is stable for one year from the date of shipment.
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