S-tag (E15)+HRP Antibody-Delta Biolabs

Product Info
Background	S-tag is an epitope tag composed of a 15 residue peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A. Frederick Richards discovered the S-peptide/S-protein system. Ribonuclease A is split by enzyme subtilisin between amino acids 20 and 21 yielding ribonuclease S. Ribonuclease S is composed of the peptide corresponding to the first 20 amino acids, S-peptide, attached to the remainding amino acids 21-124, S-protein. Ribonuclease S has the full enzymatic activity of the parent protein and the two components, S-peptide and S-protein could be separated by reversible denaturation. The separation abolished enzymatic activity, but when the two components were mixed together again, fully active ribonuclease S is formed. The unique property of reconstituting enzymatic activity by the S-tag peptide:S-protein interaction enables sensitive quantitative measurement of any fusion protein by a simple assay.
Form	S-tag (E15)+HRP is provided as an affinity purified rabbit polyclonal antibody conjugated to HRP, raised against a peptide derived from the pancreatic ribonuclease A.
Product Details	Each vial contains 200 �g in 0.2ml of affinity purified rabbit IgG, S-tag (E15)+HRP DB116, in a buffer containing 50mM HEPES pH 7.0, 0.1 M soduim chloride, 0.2% BSA and 0.01 % Thimerosal.
Immunogen	Synthetic peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A.
Specificity	S-tag (E15)+HRP DB116 recognizes protein that contain the S-tag epitope tag.
Use	S-tag (E15)+HRP DB116 is recommended for use by western blotting, immunoprecipitation, immunohistochemistry, and ELISA. The suggested western blotting starting dilution is at 1:1000.
Storage	Store this product at 4� C, do not freeze. The product is stable for one year from the date of shipment.