|
Product Info
|
|
Background |
The Escherichia coli lacZ gene encodes the enzyme beta-galactosidase. Beta-galactosidase is a large 464 kDa tetramer when assayed by western blot under nonreducing conditions and 116 kDa monomer under reducing conditions. The beta-galactosidase activity allows for the quick determination of cells expressing the lacZ gene. The lacZ gene is used as a reporter construct for determination of transfection efficiency as well as histochemical localization following transfection of eukaryotic cells. The lacZ gene product beta-galactosidase catalyzes the hydrolysis of the substrate 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) to produce a blue color that is easily visualized with a microscope.
|
|
Origin |
Rabbits were immunized with purified beta galactosidase from Escherichia coli. Antibodies were affinity purified using purified beta-galactosidase immobilized on a solid phase.
|
|
Product Details |
Each vial contains 200 µg/ml of affinity purified rabbit IgG, beta Gal DB087 (FL2), in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
|
|
Form |
200 µg/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
|
|
Immunogen |
purified beta galactosidase from Escherichia coli
|
|
Use |
Beta-Galactosidase (FL2) DB087 is recommended for use by western blotting and immunohistochemistry. The suggested western blotting starting dilution is at 1:400.
|
|
Specificity |
Will detect beta galactosidase fusion proteins
|
|
Storage |
Store this product at 4º C, do not freeze. The product is stable for one year from the date of shipment.
|
