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Product Catalog# Quantity Unit Price Buy
beta Gal (FL2) Antibody DB087 0.200 mg/ml $210.00


For technical service please call (800) 595 1994
Product Info
Background The Escherichia coli lacZ gene encodes the enzyme beta-galactosidase. Beta-galactosidase is a large 464 kDa tetramer when assayed by western blot under nonreducing conditions and 116 kDa monomer under reducing conditions. The beta-galactosidase activity allows for the quick determination of cells expressing the lacZ gene. The lacZ gene is used as a reporter construct for determination of transfection efficiency as well as histochemical localization following transfection of eukaryotic cells. The lacZ gene product beta-galactosidase catalyzes the hydrolysis of the substrate 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) to produce a blue color that is easily visualized with a microscope. 
Origin Rabbits were immunized with purified beta galactosidase from Escherichia coli. Antibodies were affinity purified using purified beta-galactosidase immobilized on a solid phase. 
Product Details Each vial contains 200 g/ml of affinity purified rabbit IgG, beta Gal DB087 (FL2), in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Form 200 g/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Immunogen purified beta galactosidase from Escherichia coli 
Use Beta-Galactosidase (FL2) DB087 is recommended for use by western blotting and immunohistochemistry. The suggested western blotting starting dilution is at 1:400. 
Specificity Will detect beta galactosidase fusion proteins 
Storage Store this product at 4 C, do not freeze. The product is stable for one year from the date of shipment. 

For Technical service please call +1-800-595-1994
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